Non-targeted metabolomics analysis reveals the mechanism of arbuscular mycorrhizal symbiosis regulating the cold-resistance of Elymus nutans

Elymus nutans is a perennial grass of the Gramineae family. Due to its cold-resistance and nutrition deficiency tolerance, it has been applied ecological restoration degraded alpine grassland on Qinghai-Tibet Plateau. As an important symbiotic microorganism, arbuscular mycorrhizal fungi (AMF) have proven great potential in promoting growth stress resistance grasses. However, response mechanism AMF needs be clarified. Therefore, this study, Rhizophagus irregularis was used explore regulating cold E. . Based pot experiments metabolomics, effects R. were investigated activities antioxidant enzyme metabolites roots under (15/10°C, 16/8 h, day/night). The results showed that lipids lipid molecules are highest proportion metabolites, accounting for 14.26% total metabolites. inoculation with had no significant at room temperature. can significantly change levels some other roots. Under stress, changed. Meanwhile, most these enriched pathways related plant metabolism. According correlation analysis, closely such as flavonoids lipids. In conclusion, may regulate grasses by affecting metabolism, antioxidant-related like These provide basis studying molecular